As in all statistical tests in Graph Pad Prism, the first window that will appear is your parameters window. Since we hypothesized that our data is related in the linear fashion, we want to click on the linear regression line. The third icon is for interpolating data from a standard curve. The first icon is linear regression and the second icon is nonlinear regression. You can access this tool from the menu bar on the analysis pane. In order to undertake linear regression analysis of our data, we will need to use a linear regression analysis tool.

So, what we want to do is perform a linear regression analysis on both datasets, compare the slopes of the lines against zero to confirm that there is a relationship and then compare the slopes of the two lines together to confirm that one and two are different in their actions. You can see from these data that there is a rough correlation between response and time in both datasets, but it appears that data one has a lower reaction to data two. We can view our data as a graph by clicking on the graph tab and you can see we have formatted this graph to show the data as a scatterplot, showing time on the X axis and our response units on the Y axis. As Prism will take this into account when we decide how to do our linear regression. This is because we fail to capture data at that point for one of our replicates. You will note that at several time points that we only have two replicates. Data one and data two and collected three replicates for each time point. In this example, we've used data from a fictitious experiment carried out over eight minutes. Prism allows you to analyze linear regression from either a single or multiple datasets with shared or individual X axes. I'm James Clark from Kings College London and in this short video, I'm going to run through the steps needed to undertake linear regression analysis of a dataset in Graph Pad Prism. This video is part of the Essential Statistics series, presented by Dr James Clark, from the School of Cardiovascular Medicine and Sciences at King’s College London. Format and annotate graphs of your results.Navigate the results tab of the analysis.Select the appropriate analysis choices.This video walks you through the steps required to perform linear regression analysis of a data set in Prism.

How to add trendline in graphpad prism